MANAGEMENT OF FLOWER MOTH (Cryptoblabes gnidiella Millière) WITH NEMATODES Heterorhabditis bacteriophora Poinar) IN MANGO CULTIVATION (Mangifera indica L.)

Authors

DOI:

https://doi.org/10.70577/asce.v5i1.707

Keywords:

Biological control, Entomopathogenic nematode, Mango pests, Integrated Pest Management, Ecuador.

Abstract

The flower moth (Cryptoblabes gnidiella Millière) is a quarantine pest of growing economic importance for mango (Mangifera indica L.) production and export in Ecuador. The use of entomopathogenic nematodes (EPN) as biological control agents is emerging as a sustainable strategy within Integrated Pest Management (IPM). This study evaluated the efficacy of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora Poinar as a biological control agent against the flower moth. The study was conducted at the Hacienda “La Frutita” during mango flowering (July–August 2024) under a completely randomized design with repeated measures over time. Five treatments were evaluated: control (no application), commercial insecticide (Mimic®), and three doses of EPN (60, 80, and 100 million infective individuals per liter), with four replicates. The effect of the applications made by foliar spraying was evaluated, and the number of parasitized larvae was quantified 24, 48, and 72 hours after application. Statistical analysis using ANOVA of repeated measures over time showed highly significant differences between treatments (p < 0.0001), with a large effect size (GES = 0.61). The results showed that H. bacteriophora achieved parasitism levels above 50% in most treatments, with the 100 million infective/L dose standing out with 90% efficacy. No interaction between treatment and time was observed, indicating a rapid and stable response in pest control. These findings suggest the potential of the nematode as an effective alternative in integrated pest management programs in mangoes, at a dose of 100 million infective larvae/L as the optimal application protocol.

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Published

2026-02-25

How to Cite

Plúas-Suárez , M. E., & Prado Beltrán, J. K. (2026). MANAGEMENT OF FLOWER MOTH (Cryptoblabes gnidiella Millière) WITH NEMATODES Heterorhabditis bacteriophora Poinar) IN MANGO CULTIVATION (Mangifera indica L.). ANNALS SCIENTIFIC EVOLUTION, 5(1), 2431–2444. https://doi.org/10.70577/asce.v5i1.707

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