Evaluación del Efecto Combinado de Coenzima Q10 y L-Carnitina sobre la Crioconservación de Semen Ovino

Ibel Carolina Guamarrigra Sanchez; Juan Carlos Alvarado Alvarado; Andrés Leonardo Moscoso Piedra; Manuel Esteban Maldonado Cornejo

doi:10.70577/ASCE/1793.1811/2025

Authors

Ibel Carolina Guamarrigra Sanchez Universidad Católica de Cuenca https://orcid.org/0009-0001-0481-8825
Juan Carlos Alvarado Alvarado Universidad Católica de Cuenca https://orcid.org/0000-0002-7240-179X
Andrés Leonardo Moscoso Piedra Universidad Católica de Cuenca https://orcid.org/0000-0002-4017-0165
Manuel Esteban Maldonado Cornejo Universidad Católica de Cuenca https://orcid.org/0000-0002-1507-2280

DOI:

https://doi.org/10.70577/ASCE/1793.1811/2025

Keywords:

Animal Genetics; Biochemical; Fertility; Samples; Veterinary Science.

Abstract

One of the main limitations for cryopreservation of ram semen as part of veterinary science is its high sensitivity to reactive oxygen species (ROS). Spermatozoa are constantly exposed to this damage due to changes in temperature, light, and their own biochemical nature, which affects their viability, motility, and functionality. This highlights the need to use additives with antioxidant properties, given their apparent positive impact on the preservation of animal genetics. The objective of this study was to evaluate the combined effect of adding Coenzyme Q10 (0.05 mM), a widely used antioxidant in reproductive supplementation, and L-Carnitine (1 mM), an amino acid derivative with functions in fatty acid transport and antioxidant activity, to the freezing medium. For this purpose, eight semen samples were collected from a single ram, and the treatments included CoQ10 (0.05 mM), L-Carnitine (1 mM), both molecules combined at the same doses, and a control (Tryladil). Motility, viability, membrane integrity and functionality, as well as mitochondrial activity, sperm progressivity, and kinematics were assessed using the C.A.S.A. (Computer Assisted Sperm Analysis System). The results showed negative effects (p<0.05) of the combined solution on all sperm quality parameters compared to the control and L-Carnitine alone, while no significant differences (p>0.05) were observed in sperm kinematics. It is therefore concluded that the combined addition of L-Carnitine and CoQ10 to the freezing medium alters the redox balance at the sperm level and reduces fertility potential.

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References

Adami, L., Maciel Júnior, V., & De Agostini Losano, J. (2022). Review on the role of antioxidant supplementation against oxidative stress: A human and animal approach to male fertility. Research, Society and Development, 11(1). https://doi.org/10.33448/rsd-v11i1.25191 DOI: https://doi.org/10.33448/rsd-v11i1.25191

Aitken, R. J., & Drevet, J. R. (2020). The importance of oxidative stress in determining the functionality of mammalian spermatozoa: A two-edged sword. Antioxidants, 9(2), 111. https://doi.org/10.3390/antiox9020111 DOI: https://doi.org/10.3390/antiox9020111

Akhigbe, T., Fidelis, F., Adekunle, A., Ashonibare, V., Akorede, B., Shuaibu, M., ... Akhigbe, R. (2025). Does coenzyme Q10 improve semen quality and circulating testosterone level? A systematic review and meta-analysis of randomized controlled trials. Frontiers in Pharmacology, 15. https://doi.org/10.3389/fphar.2024.1497930 DOI: https://doi.org/10.3389/fphar.2024.1497930

Arbulu, A. A. (2019). Nuevas estrategias para la criopreservación de esperma ovino mediante el uso de antioxidantes, gradientes y vitrificación [Tesis doctoral, Universidad de Córdoba]. Repositorio UCOPress. http://hdl.handle.net/10396/19024

Arroyo, E., Bayly, W., Chakraborty, S., Leadon, D., & Tibary, A. (2024). Effects of a semen extender treated with coenzyme Q10-ubiquinol on the quality of thawed ram sperm. Clinical Theriogenology, 16. https://doi.org/10.58292/CT.v16.10820 DOI: https://doi.org/10.58292/CT.v16.10820

Asaduzzaman, M., & Farida, Y. B. (2024). The current and advanced situation of ram semen quality in Bangladesh. Veterinary Integrative Sciences, 23(3), 1–15. https://doi.org/10.12982/VIS.2025.063 DOI: https://doi.org/10.12982/VIS.2025.063

Chavoshi Nezhad, N., Vahabzadeh, Z., Allahveisie, A., Rahmani, K., Raoofi, A., Rezaie, M. J., ... Partovyan, M. (2021). The effect of L-carnitine and coenzyme Q10 on sperm motility, DNA fragmentation, chromatin structure and oxygen free radicals during, before and after freezing in oligospermic men. Urology Journal, 18(3), 330–336. https://doi.org/10.22037/uj.v18i03.6400

Dos Santos, G., Tamanini, M. C., Leal, L. A., Wolf, L. M., Christ, T. S., Piton, Y. V., ... Mellagi, A. G. (2024). L-cysteine improves boar semen motility at 5 ºC but does not affect the oxidative status. Animal Reproduction Science, 260, 107384. https://doi.org/10.1016/j.anireprosci.2023.107384 DOI: https://doi.org/10.1016/j.anireprosci.2023.107384

Dutta, S., Sengupta, P., Roychoudhury, S., Chakravarthi, S., Wang, C., & Slama, P. (2022). Antioxidant paradox in male infertility: "A blind eye" on inflammation. Antioxidants, 11(1), 167. https://doi.org/10.3390/antiox11010167 DOI: https://doi.org/10.3390/antiox11010167

Gardela, J., Ruiz-Conca, M., Palomares, A., Olvera-Maneu, S., García-Calvo, L., López-Béjar, M., ... Álvarez-Rodríguez, M. (2023). Effect of honey, coenzyme Q10, and β-carotene/α-tocopherol as novel additives in rabbit-sperm cryopreservation extender. Animals, 13(14). https://doi.org/10.3390/ani13142392 DOI: https://doi.org/10.3390/ani13142392

Guiracocha-Viñanzaca, I., Moscoso-Piedra, A., & Alvarado-Alvarado, J. (2025). Efecto de microdosis de coenzima Q10 en la refrigeración de semen ovino previo a la congelación. MQRInvestigar, 9(1), e114-114. https://doi.org/10.56048/MQR20225.9.1.2025.e114 DOI: https://doi.org/10.56048/MQR20225.9.1.2025.e114

Hafez, B., & Hafez, E. S. (2000). Reproducción e inseminación artificial en animales (7.ª ed.). McGraw-Hill Interamericana.

Henkel, R., Sandhu, I. S., & Agarwal, A. (2019). The excessive use of antioxidant therapy: A possible cause of male infertility? Andrologia, 51(1), e13162. https://doi.org/10.1111/and.13162 DOI: https://doi.org/10.1111/and.13162

Iliceto, M. (2025). The impact of L-carnitine on human semen quality upon cryopreservation, its association with sperm fatty acids, and assessment of a novel analytical tool for DNA fragmentation in mammals [Tesis doctoral, Oslo Metropolitan University]. https://oda.oslomet.no/oda-xmlui/handle/11250/3177133

Kaltsas, A. (2023). Oxidative stress and male infertility: The protective role of antioxidants. Medicina, 59(10). https://doi.org/10.3390/medicina59101769 DOI: https://doi.org/10.3390/medicina59101769

Khosravizadeh, Z., Khodamoradi, K., Rashidi, Z., Jahromi, M., Shiri, E., Salehi, E., & Talebi, A. (2022). Sperm cryopreservation and DNA methylation: Possible implications for ART success and the health of offspring. Journal of Assisted Reproduction and Genetics, 39(8), 1815–1824. https://doi.org/10.1007/s10815-022-02545-6 DOI: https://doi.org/10.1007/s10815-022-02545-6

Kowalczyk, A. (2022). The role of the natural antioxidant mechanism in sperm cells. Reproductive Sciences, 29, 1387–1394. https://doi.org/10.1007/s43032-021-00795-w DOI: https://doi.org/10.1007/s43032-021-00795-w

Lançoni, R., Celeghini, E., Giuli, J. V., Carvalho, C., Zoca, G. B., García-Oliveros, L. N., ... Arruda, R. P. (2021). Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm. Animal Reproduction, 18(1). https://doi.org/10.1590/1984-3143-AR2020-0218 DOI: https://doi.org/10.1590/1984-3143-ar2020-0218

Mantle, D., & Dybring, A. (2020). Bioavailability of coenzyme Q10: An overview of the absorption process and subsequent metabolism. Antioxidants, 9(5), 386. https://doi.org/10.3390/antiox9050386 DOI: https://doi.org/10.3390/antiox9050386

Masoudi, R., Sharafi, M., & Shahneh, A. (2019). Effects of CoQ10 on the quality of ram sperm during cryopreservation in plant- and animal-based extenders. Animal Reproduction Science, 208, 106128. https://doi.org/10.1016/j.anireprosci.2019.06.015 DOI: https://doi.org/10.1016/j.anireprosci.2019.06.015

Mateus, F. G., Moreira, S., Martins, A. D., Oliveira, P. F., Alves, M. G., & Pereira, M. L. (2023). L-carnitine and male fertility: Is supplementation beneficial? Journal of Clinical Medicine, 12(18). https://doi.org/10.3390/jcm12185796 DOI: https://doi.org/10.3390/jcm12185796

Njoroge, W. E., Zhu, Z., Umehara, T., Yamanaka, T., Zeng, W., Okazaki, T., & Shimada, M. (2025). Synthesis of functional enzymes involved in glutathione production during linear motility in boar sperm. Free Radical Biology & Medicine, 228, 126–136. https://doi.org/10.1016/j.freeradbiomed.2024.12.051 DOI: https://doi.org/10.1016/j.freeradbiomed.2024.12.051

O’Flaherty, C., & Scarlata, E. (2022). Oxidative stress and reproductive function: The protection of mammalian spermatozoa against oxidative stress. Reproduction, 164(6), F67–F78. https://doi.org/10.1530/REP-22-0200 DOI: https://doi.org/10.1530/REP-22-0200

Puerta, M., Henao-Salazar, L., Vélez, I., León, S., Rojano, B., Restrepo, G., & Úsuga, A. (2024). Dietary antioxidant supplementation improves the in vitro quality and antioxidant capacity of Colombian Creole stallion semen. Czech Journal of Animal Science, 69(11), 450–461. https://doi.org/10.17221/98/2024-CJAS DOI: https://doi.org/10.17221/98/2024-CJAS

Quinche-Guazhambo, M., Moscoso, A., Cornejo, M., & Alvarado, J. (2025). Microdosis de coenzima Q10 como potencializador de la calidad espermática de semen ovino crioconservado. Ciencia Latina Revista Científica Multidisciplinar, 9(1), 10059–10074. https://doi.org/10.37811/cl_rcm.v9i1.16625 DOI: https://doi.org/10.37811/cl_rcm.v9i1.16625

Ribeiro, J., Braga, P., Martins, A., Silva, B., Alves, M., & Oliveira, P. (2021). Antioxidants present in reproductive tract fluids and their relevance for fertility. Antioxidants, 10(9), 1414. https://doi.org/10.3390/antiox10091441 DOI: https://doi.org/10.3390/antiox10091441

Sotler, R., Poljšak, B., Dahmane, R., Jukić, T., Pavan Jukić, D., Rotim, C., ... Starc, A. (2019). Prooxidant activities of antioxidants and their impact on health. Acta Clinica Croatica, 58(4), 726–736. https://doi.org/10.20471/acc.2019.58.04.20 DOI: https://doi.org/10.20471/acc.2019.58.04.20

Valdez-Pinargote, G., Moscoso-Piedra, A., & Maldonado-Cornejo, M. (2024). Efecto de la concentración de la L-carnitina en congelación del semen ovino. MQRInvestigar, 8(4), 7324–7341. https://doi.org/10.56048/MQR20225.8.4.2024.7324-7341 DOI: https://doi.org/10.56048/MQR20225.8.4.2024.7324-7341

Vicente-Fiel, S., Palacín, I., Santolaria, P., Fantova, E., Quintín-Casorrán, F. J., Sevilla-Mur, E., & Yániz, J. L. (2014). In vitro assessment of sperm quality from rams of high and low field fertility. Animal Reproduction Science, 146(1–2), 15–20. https://doi.org/10.1016/j.anireprosci.2014.02.005 DOI: https://doi.org/10.1016/j.anireprosci.2014.02.005

Wang, Y., Fu, X., & Li, H. (2025). Mechanisms of oxidative stress-induced sperm dysfunction. Frontiers in Endocrinology, 16. https://doi.org/10.3389/fendo.2025.1520835 DOI: https://doi.org/10.3389/fendo.2025.1520835

Yang, S., Fan, B., Chen, X., & Meng, Z. (2021). Supplementation of the freezing medium with coenzyme Q10 attenuates oxidative stress and improves function of frozen-thawed giant grouper (Epinephelus lanceolatus) spermatozoa. Theriogenology, 175, 77–82. https://doi.org/10.1016/j.theriogenology.2021.08.029 DOI: https://doi.org/10.1016/j.theriogenology.2021.08.029

Zhang, X., Liu, Q., Wang, L., Yang, G., & Hu, J. (2016). Effects of glutathione on sperm quality during liquid storage in boars. Animal Science Journal, 87(10), 1195–1201. https://doi.org/10.1111/asj.12545 DOI: https://doi.org/10.1111/asj.12545